Panãra, an Indigenous language native to Brazil, is currently the focus of Dr. Myriam Lapierre, Sunkulp Ananthanarayan, Ella De Falco, and Jessamine Jeter as some of the only linguists to document and conduct a comprehensive study on this language. Our research focuses on streamlining the process of organizing and analyzing field data – specifically in the context of Panãra, though generally applicable to other Indigenous and/or under-researched languages  – for use in future research by Dr. Lapierre and other scholars in the field of linguistics as it applies to Indigenous and minoritized languages. We have digitized the data from the field journals of Dr. Lapierre and the graduate students working with her, and our current focus is on the analysis of verb and sentence construction, via this digitized data, to organize grammatical paradigms into efficient and accessible indexes. We are also compiling and organizing PDF, image, sound, video, and experimental data for use on the California Language Archive (CLA) with a similar focus on efficiency and accessibility. The completion of this research entails the more complex understanding and organization of Panãra sentence and word structure for use in future research, both by Dr. Lapierre and by other scholars, as well as for usage in a Panãra dictionary. Our expected results also involve the creation and organization of the CLA page dedicated to Panãra, with a transparent structure making this data available to a wider audience of both linguists and non-linguists interested in learning more about the language.

Meningiomas, the most common type of primary human brain tumor, arise from the thin fibrous membrane that covers the brain and spinal cord. Most grow slowly and are diagnosed when they disrupt brain function or lead to persistent headaches. While many meningiomas can be cured by surgery, ~20% of them cannot be fully resected or display increased growth, invasion and destruction of adjacent brain and skull. Effective control or eradication of these ‘High Grade II/III’ meningiomas is clinically challenging. To identify new agents and treatment measures, our project uses both computational and experimental approaches in concert to identify new and potentially better therapies. As part of this effort, we are using PISCES, a machine learning model, together with augmented drug and radiation combination datasets to predict potential new therapy synergies. The best predictions from PISCES will then be tested experimentally in our cell line model versus standard-of-care treatments.  My presentation summarizes work to characterize genomic, drug and ionizing radiation sensitivity data on IOMM-Lee, a Grade III human meningioma cell line disease model. We detail how A.I.-driven analyses of IOMM-Lee and related meningioma datasets led us to test new drug pairs and drug-radiation combinations predicted by PISCES to be more effective in killing IOMM-Lee tumor cells. This translational cellular disease model and project are part of a long-term effort to develop better ways to rapidly and efficiently identify and validate new treatment options for brain tumors and other human cancers that can be taken directly to clinical trial.

Current Gravitational-Wave observatories mainly focus on gravitational wave(GW) detection at frequency bands below 10kHz, probing signals that are expected to arise from known astrophysical sources, leaving frequency ranges above 10kHz largely unexplored. GWs with frequencies beyond 10kHz correspond to Ultra-High-Frequency Gravitational Waves(UHF-GW) that are theorized to be sourced by various Beyond-the-Standard-Model(BSM) phenomena both in the early and late universe, providing an unique window to probe for new physics. The Axion Dark Matter eXperiment(ADMX) is a resonant cavity designed to search for axion dark matter, however, recent studies have shown that resonant cavities like ADMX have a possible sensitivity towards UHF-GW within the GHz range through GW-Electromagnetic coupling. In our research, we provide a detailed examination of ADMX’s experimental sensitivity with regards to the strain of UHF-GW generated by two hypothetical sources: primordial black hole(PBH) binaries and boson annihilation within boson clouds generated by black hole superradiance. We investigate various source parameters, such as GW strain amplitude, GW signal duration time, and the merging rate of PBH binaries, to determine ADMX’s detection capability for potential GW source candidates. Our work will provide ADMX with insights on the plausibility of monochromatic UHF-GW detections with current experimental parameters and serve as a motivation towards inquiries of alternative UHF-GW sources or imminent data analysis strategies depending on the outcome.

The purpose of this study is to understand recent developments regarding curriculum standards for teaching Indigenous histories and contemporary matters, specifically within Michigan’s K-12 Public Schools(MPS). This presentation draws from interviews with a range of stakeholders, including: current and former students and educators within MPS; politicians and government officials involved in relevant legislation; university professors concentrating their work in Native Studies at universities in Michigan; and representatives of Tribal Nations located in Michigan. This project also integrates relational discussions with Washington-based educators involved in the teaching and implementation of Washington’s Since Time Immemorial (STI) Curriculum. This presentation will share research analyses of relevant pieces of legislation and academic sources pertaining to Indigenous-centered curriculum. The objective of this research is to inform a written piece addressing current efforts to expand education on Indigenous histories and contemporary matters in MPS including efforts made in the past, actions currently in progress, suggested plans for the future, and what Michigan may learn from Washington’s efforts to fully implement STI through examining shortcomings in the implementation of STI curriculum and how these failures may serve to inform Michigan’s protocol for introducing revised standards. Given that one of the major proposals to the expansion of Indigenous-related curriculum involves teaching Indian Boarding School histories, the long-term implications of this research contribute to ensuring youth are educated about the devastating consequences of residential schools, which in turn aims to assure similar policies are not introduced in the future. Discussion of these findings will emphasize institutions which allow(ed) destructive policies like that of boarding schools to be implemented, reframing the common narrative perpetuated in schooling systems that such policies are the result of a few “bad actors.” This research is interested in exploring how curricula contribute to the latter perspective, and whether newly implemented standards effectively convey the former viewpoint.

Ferrate is an effective technology for water treatment applications because of its capabilities as an oxidant, coagulant, and disinfectant. Furthermore, ferrate is an environmentally benign chemical derived from a ubiquitous mineral on the Earth’s surface. However, ferrate rapid reduction to ferric species reduces its oxidation capacity. Ferrate-coated sand has been proposed as a better deployable method for ferrate in water treatment applications. Sand has a high composition (>80%) of silica (SiO₂) which has been demonstrated to stabilize ferrate reactivity and increase its oxidation capacity. A previous study on the treatment of phenol, a common surface water contaminant, showed that ferrate-coated sand was better at degrading phenol than ferrate only (in the absence of sand). However, the study was conducted in pure water matrices. Here, we are evaluating the oxidation of phenol by ferrate-coated sand in the presence of effluent organic matter and trace metals (i.e. copper). Organic matter is ubiquitous in the environment and can impact contaminant remediation efficiency. Studies have detected trace metals in surface waters which can pose environmental and health risks. Through batch tests, we observed that effluent organic matter hinders the stability of the ferrate-coated media and reduces its oxidation capacity. The results of this study will provide information about the ferrate-coated sand reactivity and capacity for the treatment of complex water matrices.

Vertical velocities are a fundamental component of ocean flow and are vital to characterizing global circulation. However, vertical velocities are small compared to horizontal velocities and are thus difficult to measure. Previous studies attempting to estimate them ignore the impacts of topography, mesoscale eddies, internal waves, and spatial variability. Novel estimates from the Argo float array allow for direct estimates of vertical velocities. This project will focus on comparing these new Argo estimates with vertical velocity observations from moorings in the Southern Ocean. The Southern Ocean is an important site of vertical volume transport for mass ocean circulation with global implications, particularly the Antarctic Circumpolar Current which dynamically links many of these interactions. We expect vertical velocity characterized by moorings to maintain coherency with Argo float estimates. Differences may occur, however, due to mismatches in spatial resolution between Argo-based estimates and mooring-based estimates, which rely on mass conservation across larger scales. In comparing novel Argo datasets to known mooring values, we gain a more complete understanding of vertical velocities in the Southern Ocean which have direct implications for data assimilation in models and parameterization of energy pathways.

Using data from the United States Sentencing Commission (2014-2022), this study analyzes the impact of a defendant's gender in the sentencing of federal homicide cases. Previous research shows that female defendants experience leniency in criminal sentencing compared to male counterparts. However, studies also suggest harsher punishments are given to female defendants when the crime is violent. This may be influenced by the social construct of gender, where traits including aggression and violence are perceived to be inherently male, and male violence is often expected and excused. Focusing exclusively on federal homicide cases from fiscal years 2014-2022 (N = 3017), I ran linear regression analyses controlling for legal and extralegal factors to analyze the effect of gender on sentence length. I hypothesize that in the case of federal homicide, male defendants will receive shorter sentences compared to female counterparts. This study focuses how gender influences sentencing outcomes in federal courts. It is crucial to understand how gender influences judicial sentencing in order to promote a just legal system.

Alzheimer’s disease (AD) is the most common neurodegenerative disease, with over 6 million Americans suffering from the illness and prevalence increasing each year. My work was conducted as part of an NIH-funded multi-institutional network called TREAT-AD (TaRget Enablement to Accelerate Therapy Development for AD) that aims to find potential therapies for AD. The bioinformatics core of this network identified genetic targets of interest using RNA-sequencing and proteomic analysis of post-mortem tissue from participants with AD. We hypothesized that manipulating expression of these target genes in a relevant human model would influence levels of AD-related biomarkers. To manipulate genetic expression efficiently, I used shRNA technology in human induced pluripotent stem cell (hiPSC) derived neurons. I then analyzed these hiPSC-derived neurons for AD-relevant readouts, such as soluble amyloid beta secretion and intracellular phosphorylation of Tau protein, relevant to the two main neuropathological hallmarks of AD. I ran quantitative polymerase chain reactions (qPCR) to measure neuronal expression levels of each gene target, and compared amyloid beta and phosphorylated tau outputs to control samples using MSD ELISA assays. I found four gene targets that have substantial neuronal expression and found that each affected AD-related output levels when gene expression was knocked down with shRNA. My findings provide direct molecular genetic evidence that links these genes to AD pathways, suggesting that these genes could serve as promising targets for therapeutic development.

Collision avoidance studies find important applications for motion planning of mobile robots for deployment in outer space, nuclear waste management, mobiles used for process automation, etc. Here, we integrate mobile robot simulations with mathematical modeling using Python to understand collision avoidance for mobile robotics. We used the open-source Pioneer code on the Webots platform for simulations of mobile robots which employ Kinect-based optical and IR sensors and cameras for live-tracking of objects in the environment variable and have motion controller Matlab software that provides the kinematic variables like position, velocity, and acceleration of various objects in real-time. We wrote a Python code to digitize the image matrices obtained from simulations and identified the pixels having objects that the mobile robot must avoid for collision avoidance. We calculated the instantaneous distances between the mobile robot and various objects to interpret and analyze the simulated trajectories. We used jump collision avoidance models to estimate the mobile robot trajectories in the vicinity of objects. The calculated object avoidance jump trajectory of the robot was smoothened using Gaussian data convolution methods to obtain smooth trajectories. The simulations provide attractive visualization and are useful for machine learning and testing algorithms for collision avoidance and motion planning.

6PPD-quinone (6PPDQ), a transformation product of an anti-oxidant used in tire manufacturing, was recently identified as the causal agent of acute mortality in coho salmon. Abrasion on tires by road surfaces create tire wear particles (TWPs). Both TWPs and the accumulation of waste tires pose risks of leaching 6PPDQ into stormwater runoff. Crumb rubbers, which are manufactured to reduce landfill tire waste and applied in turf infills, may also leach 6PPDQ. My research aims to determine the conditions at which crumb rubber can be pyrolyzed to prevent 6PPDQ leaching from tire recycling options. If pyrolysis successfully removes 6PPDQ from crumb rubber, then the resulting material can be applied as an absorbent tire char to remove contaminants from water. Waste tire crumb rubber samples were pyrolyzed in a tube furnace under nitrogen flow for 90 minutes at a range of different temperatures. Methanol-based solvent extraction was used to extract the remaining 6PPDQ from the pyrolyzed samples and diluted until suitable for liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis. It is observed that as the pyrolysis temperature increases, the mass of 6PPDQ leached from pyrolyzed crumb rubber decreases. The results of this study allow us to understand the limitations of pyrolyzing tire rubber to develop activated carbon. To further investigate the feasibility of waste tire activated carbon, a chemical activation step will be added in pyrolysis to better replicate the creation of activated carbon.

Respiratory viruses are a major cause of mortality and morbidity in vulnerable populations. Together, Respiratory syncytial virus (RSV) and Human metapneumovirus (HMPV), are responsible for over â…“ of serious viral respiratory infections in hematopoietic stem cell transplant (HCT) recipients. Currently, no treatments are available for RSV or HMPV in immunocompromised adults. While monoclonal antibodies (mAbs) show promise as a treatment, challenges arise, including limited efficacy when administered post-infection. Our goal was to enhance the therapeutic efficacy of a newly discovered cross-neutralizing human mAb to RSV and HMPV. We aimed to investigate whether modifying the Fc domain of the antibody could increase its binding to Fcγ receptors (FcγRs) found on different types of immune cells. Activation of FcγRs initiates important cell processes such as clearance of virus-infected cells, also known as Antibody-dependent cellular cytotoxicity (ADCC). This modification potentially makes the antibody a more effective treatment option for RSV and HMPV infections. To do this we looked at the binding kinetics and affinity of modified antibodies to human FcγRIIIa, FcγRIIa and FcγRIIb receptors using Bio-Layer Inferometry (BLI). Our data indicate that certain amino acid modifications or afucosylation of the Fc region can increase the antibody’s binding affinity to different human FcγRs. Since hamsters are an important preclinical model used to determine RSV and HMPV drug efficacy, it was important to examine the binding affinity of our human antibody to hamster FcγR’s. Our data indicate that the wild-type Fc region does bind to the homologous hamster receptors. Moreover, certain modifications in the Fc region led to increased binding to hamster FcγR’s. Together, these data indicate that modifications in the Fc region of human antibodies can increase their binding affinity to both human and hamster FcγRs. This increase in binding affinity could translate to enhanced potency in the preclinical hamster model and in humans.

Examining environmental exposures during pregnancy and their impacts on fetal and child development is vital for maternal and child health. As researchers, we have a responsibility to return these individual environmental results to participants. DERBI (Digital Exposure Report-Back Interface) is an online tool to specifically return environmental exposure results. This study examines: Are there distinguishable demographic-based patterns in those who logged in to open their results versus those who did not? What demographics are we missing in getting participants their results? PATHWAYS GAPPS is an NIH-funded study that examines environmental exposures and child outcomes in Seattle and Yakima, Washington. DERBI provided personalized biomonitoring results for environmental chemicals after over a decade of participation in research. Three hundred ninety-three participants across the Seattle (N=194) and Yakima (N=199) sites were sent an access code for their child’s return of results. I compiled demographic data on socioeconomic factors, such as race and maternal education. Within each site, I analyzed the demographics on which participants had opened their child’s return of results and those who did not open them. I then calculated and rounded percentages over the opening status for each site. At the point of data collection, mothers had five months to log in and open their results. I observed that mothers with less education were less likely to open their results, and fewer non-Hispanic Black mothers opened their results, suggesting that education and self-identified race are key factors in determining who is likely to access their results. The manner we present the return of results to participants and provide additional resources to process and take action on the results also matters. Future directions include conducting qualitative interviews and focus groups to hear first-hand from participants about barriers in accessing results and what resources would increase inclusivity and likelihood of opening their results.

Pulmonary Arterial Hypertension (PAH) is a deadly vascular disease, affecting the blood vessels of the lungs rather than the systemic circulation, with no existing cure. PAH is characterized by pulmonary arterial smooth muscle cell (PASMC) hypertrophy and hyperplasia, which increases resistance to blood flow within the pulmonary arteries and leads to rapid symptom progression and death from right heart failure over several years. We hypothesize that defects in PASMC differentiation and alignment may contribute to PAH. Prior work has shown that micropatterned scaffolds encourage vascular SMC alignment and differentiation towards a contractile phenotype. To test whether these responses differ in patients with PAH, we designed a micropatterned collagen scaffold atop a glass coverslip. Scaffoldings were imprinted with either alternating 10-µm wide x 10-µm deep microchannels or left unpatterned. Explanted PASMCs from patients with PAH or failed donors (controls) were cultured on patterned versus unpatterned constructs and alignment, protein expression, and cellular morphology were compared across conditions. I evaluated 3 PAH and 3 control subjects and have collected preliminary data for each condition (control versus PAH), with three technical replicates each. Through these preliminary studies, I have demonstrated success of my model with consistent alignment observed on patterned substrates. Excitingly, PASMCs from patients with PAH expressed significantly decreased levels of the contractile protein, Calponin, when compared with control cells, including after responding to cues that promote alignment and contractility. This suggests that PAH PASMCs remain in an inappropriately synthetic or proliferative state. Subsequent testing will include assessment of calcium signaling in response to contractile stimuli and transcriptomic evaluation of cellular responses to micropatterning. This work will enhance understanding of whether SMC abnormalities contribute to disease initiation and progression in PAH and will contribute to the broader effort of developing more complex models of pulmonary vascular disease.

Recent findings suggest that decreased chromatin acetylation leads to neurodegenerative diseases including Alzheimer's Disease (AD). Histone acetylation alters chromatin structure which regulates gene expression. One of the key proteins involved in this genetic modification is KAT5 (TIP60) acetylase. The Young Lab recently demonstrated that increased chromatin acetylation leads to the expression of genes involved in cognition and neuronal maturation. KAT5 is known to interact with the intracellular region of the Amyloid Precursor Protein (APP), which is a main player in the development of Alzheimer’s Disease (AD). In wild-type cells, the KAT5 protein is believed to be released from the membrane and translocated to the nucleus where it leads to increased chromatin acetylation and gene expression. Recent hypothesis suggest that an amyloidogenic cleavage of APP, either due to mutations or in AD conditions, prevents KAT5 nuclear translocation. To address this question, we will perform the following three experiments using human brain tissue and human induced pluripotent stem cell-derived neurons (hiPSC-Ns): 1. Immunohistochemistry for KAT5 on control and AD brains to see if we can detect a reduction in nuclear KAT5 localization in AD. 2. Immunocytochemistry on hiPSC-derived neurons harboring a familial AD (fAD) mutation in APP (Swedish mutation-K670N/M671L) as well as in neurons derived from cells that are deficient in APP (APP KO). 3. RNA-sequencing to determine differential gene expression between cells with fAD mutations and those that are deficient in APP, with a focus on genes regulated by KAT5. We expect to see aberrant KAT5 localization and gene expression in cells and tissues with AD and fAD mutations. Since treatments targeting the deposition of beta-amyloid led to many unsuccessful medical trials, we anticipate this study will demonstrate the importance of the absence of KAT5 signaling during early development of AD and devise new strategies for treatments.

Sound symbolism is a phenomenon wherein the phonetic forms of certain words iconically represent attributes of the objects, qualities, or events they describe. This study focuses on the sound-meaning link in Panãra animal names, using vocabulary from field notes collected in the Panãra community by Dr. Lapierre the summers of 2015-19 and by Dr. Lapierre and PhD students Ananthanarayan, De Falco, and Jeter the summer of 2023. The Panãra vowel system has a combination of features not present in some more widely studied languages such as English, namely, a back, unrounded series, contrastive length, and contrastive nasality. Using this extensive inventory, I assess strength of size sound symbolism created by nasality, height, vowel length, and backness, as well as the interactions between multiple features. I organize Panãra names for different animals and find the average weight of that species. I calculate the percentage of vowels that have a certain feature in a word and assess the correlation between this percentage and the weight of the species denoted using a regression model. Previous research has shown that front and high vowels are associated with smaller sizes and back and low vowels with larger sizes. I predict that the phonetically central and mid series will be associated with sizes intermediate to the peripheral series. Alongside providing observations from an under-documented language regarding its sound symbolism, findings from this study will help guide the continued lexicographic and field research inquiries into the Panãra language.

Plainfin midshipman (Porichthys notatus) are seasonally reproducing teleost fish found along the Pacific Coast of North America. During the non-reproductive months (December-February), midshipman live offshore in deep waters. Between March and July, they migrate to the intertidal zone to reproduce. During the reproductive period, dominant (type I) males build and defend nests and hum to attract female mates. Unlike most vertebrates, courtship (humming to attract mates) and parental care (egg cleaning, nest defense) are both conducted only by type I males. These males therefore face energetic and temporal trade-offs between courtship to parental care within a single reproductive season. Our research investigates the neural mechanisms underlying the transition from courtship to paternal care in type I males during the reproductive period. We propose that this behavioral shift is regulated by steroid hormones (i.e. testosterone and estrogen) and neuropeptides (i.e. galanin). We are measuring hormone levels in blood and brain of midshipman fish across various conditions of courtship and parental care. We show the relationship between changes in steroid and neuropeptide levels in both blood and brain and changes in courtship and parental care behavior in type I males. Understanding these mechanisms in midshipman fish is crucial, as the steroid and neuropeptide pathways that regulate social behaviors share similar pathways across vertebrates. Therefore, studying this in teleost fish can provide valuable insights into the broader regulatory mechanisms of reproductive life-stage transitions in vertebrates.

Smartphone use among adolescents has dramatically increased in the past decade, with adolescents allocating more time inside on their screens and less time outside in nature. Given these shifts in behaviors, there has been a rise in concerns about the impact of smartphones on adolescents’ mental health. Studies on nature exposure have found that spending time in nature promotes human well-being; however, a limited body of research exists exploring how smartphone use impacts our ability to experience these physical and psychological benefits of nature exposure. To address this gap in the literature, we conducted a between-subjects experiment with college students at the University of Washington (N ~ 40) randomly allocated to one of two conditions to spend 20 minutes in nature: a smartphone condition and a no-smartphone condition. Participants self-reported on positive and negative affect and rumination before and after their experience in nature. After completing these scales, participants reported on their experience of Presence, a state of being in which the mind is highly aware but without active thought, by self-reporting on a recently validated 14-point scale and writing about it for further analysis using an interaction pattern approach. I anticipated that participants who feel more attached to their smartphones spend more time using them in nature and, in turn, are less likely to experience the emotional benefits of nature. If the findings reflect this, future research should explore how smartphones impact adolescents’ mental health and the extent to which smartphone-free time spent outside in nature can improve their well-being.