The delivery of cell and drug-based chemotherapeutics to tumors have presented major challenges in effective cancer treatment. Opportunities to improve current small molecule drug delivery systems exist by increasing overall delivery specificity and decreasing harmful off-target effects. Towards this, we have recently developed a chemical framework for creating user-programmable hydrogels that undergo programmed degradation in response to multiple environmental cues following Boolean logic. Exploiting this methodology, user-specified combinations of environmental inputs (e.g., tumor-presented enzymes, reducing conditions) yield material breakdown and accompanying therapeutic release. To translate these materials for chemotherapeutic delivery in vivo, we established strategies to formulate these stimuli-sensitive materials into nanogels that circulate in the bloodstream before acting on the desired target site. We developed techniques to formulate gels on the 50-250 nanometers size scale, one which should enable circulation in the blood and uptake within tumors based on the enhanced permeability and retention effect. Different ultrasonication conditions allowed us to tune nanogel, size and dispersity. This system is scalable, translational, and simple to recreate. In the future, these materials can effectively hone and selectively deploy small molecule chemotherapeutics to tumors in patients.

Legal theorists have long maintained that courts operate beyond their primary function of dispute-resolution and have problematized their characterization as “objective” bodies, noting their significance as powerful social and political actors bestowed with constitutive powers of meaning-making. Virtually absent in this scholarship is an analytical angle examining this constitutive power using theory or methods from performance studies. This is surprising, as courtrooms are highly theatrical spaces. My research seeks to fill this gap in scholarship by marrying theories and methods from sociolegal studies and theatre- and performance studies to examine how courts contribute to the construction of cultural meanings pertaining to identity. This essay treats the European Court of Human Rights (Court), the judicial organ of the Council of Europe and one of the most active, powerful international human rights courts in the world. How does the Court construct notions of identity – especially around nationality, European community, gender identity, and religion? More specifically, my project asks: How are these courtroom constructions conceived and legitimized through narrative performance, and how is their sociopolitical influence shaped by the mechanics of performativity? To answer these questions, I conduct an original research project analyzing both written judgments and video recordings of oral hearings held in the Court’s Grand Chamber. I form my own criteria to analyze these hearings as performances, and create a scheme to evaluate written judgments for their performativity. I also analyze certain structural characteristics of the Court, and some legacies of its case law, as symbolic and embodied performances, examining how identity narratives are reproduced by the Court’s composition as an institution and its behavior over time. In addition to demonstrating what can be gained by critically assessing courts holistically using performance theory and methodology, I hope to illuminate exciting intersections between sociolegal studies and theatre- and performance studies with this work.

Precise spatiotemporal control over biochemical cue presentation is necessary to mimic the complex, heterogenous environments found in biological systems. Achieving this level of control within engineered microenvironments would allow for the manipulation of cell growth and differentiation, which could be utilized in tissue engineering and drug delivery. To this end, we developed a method that utilizes fusion proteins made from a novel PhotoCleavable protein linker (PhoCl) and a protein of interest (POI). This method allows for spatiotemporal control of POI release from hydrogels in response to cytocompatible violet light (λ = 405). This system is flexible, as PhoCl can be conjugated to many different POIs, including fluorescent proteins, enzymes, and growth factors, and was found to not affect protein function. Additionally, PhoCl undergoes a green-to-red transition after photocleavage, allowing for real-time tracking and quantification of POI release. As PhoCl cleaves in response to visible light, which is less damaging to cell function and has a greater tissue penetration depth than the traditionally used UV light, PhoCl fusion proteins hold promise for use in vivo. To demonstrate the feasibility of this system, PhoCl fusion proteins were formed with several fluorescent proteins (e.g., mRuby, sfGFP, mCerulean). Conjugating these fusion proteins into gels and exposing them to patterned light produced spatiotemporal localized release of proteins with micron scale resolution, which was demonstrated through fluorescent imaging of the photopatterned gels. To support the potential in vivo applications of this system, PhoCl was also used in mammalian cell studies with epidermal growth factor (EGF). These studies showed the expected increased cell growth in response to photomediated EGF release. This illustrates the potential versatility of the PhoCl system in biological applications, thus supporting the relevance of this novel system to tissue engineering and drug delivery methods.

Over the past several summers, Washington state has faced high levels of wildfire smoke exposure. Significant research has indicated that wildfire smoke is a public health hazard. Public health professionals require evidence-based communication and intervention methods for use in exposed communities. It is valuable for researchers to understand current practice-based risk communication and management challenges to inform their research strategy and priorities. In response, the University of Washington's School of Public Health faculty and students convened a wildfire smoke risk communication symposium on October 30th, 2018, to foster academic-practice partnerships among regional stakeholders, identify existing risk reduction and communication challenges, and identify research needs. We conducted pre and post symposium surveys to collect information on participants’ goals, demographic characteristics, and symposium satisfaction. Descriptive statistics were calculated and displayed on graphs and tables. The registration survey identified the most common participant goals for the symposium which were to learn about lessons learned from public health practitioners related to wildfire smoke and to identify collaboration opportunities. Participants had a variety of roles related to risk communication and research. The evaluation survey revealed that over half of participants reported knowledge increases on wildfire risk communication, the future of wildfires in the Pacific Northwest, and practice-based responses to wildfire smoke. Over three-quarters of participants reported developing new connections that could lead to new collaborations in the future. Our findings suggest that there is an interest in working collaboratively to improve risk communication during wildfire events to address knowledge gaps and challenges impacted communities may face. We recommend engaging stakeholders and participants to identify the best communication methods and work with multidisciplinary partnerships that will be crucial to prepare for future wildfire seasons.

This project looks to understand the role of women in the second rise of the Ku Klux Klan in the 1920s to understand why this manifestation has been categorized as the largest right-wing movement in the history of the United States. I argue that the addition of women as active participants in Klan activity, unlike the first rise during Reconstruction which was a strictly fraternal society, transformed the movement from a domestic terrorist organization into a political club with immense social influence on the white Protestant population in America. Primary sources used to build this argument came from Klan documents such as pamphlets and newsletters as well as local and national newspapers from across the U.S between 1918-1927. Women in this time period were emboldened to participate in politics after their victory with the suffrage movement, and those who employed racist and nativist ideology easily transitioned into the white supremacy of the Klan, who desperately sought to recruit blocs of voters. Using coded language such as "100% American" to describe themselves, the Women's Ku Klux Klan (WKKK) utilized issues such as poor education, alcoholism, and immigration as a silk screen to vilify their ever-growing list of "enemies" (a tool used to recruit membership from a larger base) among them Catholics, Jews, Bolsheviks, blacks, labor unions in the North, and immigrants. The addition of women allowed the Klan to become an organization that supported nuclear family structure and encouraged all to be involved, including children, which served to develop “Klan culture” to recruit and retain members by building community. While Klanswomen were different from their male counterparts, they worked within social networks that maintained consistent growth, starting chapters in almost every state and amassing political and social influence on a local and national level.

The Methow Valley is a community located in Okanogan County, WA that has experienced wildfires in 4 of the past 5 years. The Methow Valley Clean Air Project (MVCAP) is a local volunteer group that promotes air quality awareness through outreach and programming. MVCAP’s Purple Air Network was designed to provide access to spatial air quality information to help community members make decisions about protective actions, to identify relatively clean air spaces, and to serve as a public health invervention for wildfire smoke. In collaboration with MVCAP, we worked to install a network of 20 purple air monitors in the summer of 2018. The monitors were placed in homes of “Clean Air Ambassadors” who committed to maintain and promote the monitor. For calibration, each monitor was placed according to federal guidelines and two were collocated with nephelometers maintained by Washington Department of Ecology. During the summer of 2018, two wildfires burned nearby which allowed the monitors to be utilized in a wildfire smoke event. The data from the monitors was downloaded and compared to the nephelometer data using linear regression. I worked to establish a correction factor by analyzing the data and found that the Purple Air monitor over reported the PM2.5 concentration by a factor of 0.53 and even more at high concentrations. The network proved the importance of high spatial concentration monitoring by capturing the air quality variation. In some areas, the air quality was good while in others it was hazardous. Having the network is a tool for individuals to know what the air quality is near their homes and other places in their community. It allowed individuals to access local air quality data and make choices about poor air quality. The next step for MVCAP is designing an online interface that applies the correction factor directly to accurately communicate risk.

Clallam County has recently been experiencing concerning air quality conditions due to smoke drift from wildfire events in nearby areas. The objective of this study was to assess the wildfire smoke health-risk communication needs of organizations that serve the public in Clallam County. Communication needs were assessed by surveying 10 organizations that serve sensitive populations. Surveys were conducted in person or over the phone, and summary statistics were calculated. In addition, a low-cost air monitor was installed to track summer time air quality. The low-cost air monitor was installed adjacent to a nephelometer administered by the Olympic Region Clean Air Agency (ORCAA). This was done to determine the accuracy of the low-cost air monitor by comparing air quality values registered from both devices. The majority (60%) of organizations responding to the survey reported that they had not received information about the health risks of wildfire smoke. Nearly all organizations (90%) reported that they have the capacity and are willing to communicate the health-risks of wildfire smoke to the people they serve in Clallam County. Analysis of air quality data shows the low-cost monitor may be a useful device in determining air quality conditions. The correlation coefficient for the daily averages (from July 12 to August 24) between the ORCAA nephelometer and the low-cost air monitor was determined to be 0.98, but closer examination of data for hourly averages show a correlation coefficient as low as 0.82. Future wildfire smoke events in Clallam County require public health interventions to address health-risk communication needs of sensitive populations. Implementation of a low-cost air monitor network accessible by the public is a promising prospect to protect sensitive populations in Clallam County.